Intramolecular 1H–13C distance measurement in uniformly 13C, 15N labeled peptides by solid-state NMR
نویسندگان
چکیده
منابع مشابه
Accurate measurement of 13C-15N distances with solid-state NMR.
Solid-state NMR technique for measuring distances between heteronuclei in static powder samples is described. It is based on a two-dimensional single-echo scheme enhanced with adiabatic cross polarization. As an example, the results for intramolecular distances in alpha-crystalline form of glycine are presented. The measured NMR distances (13)C(alpha)-(15)N and (13)C(')-(15)N are 1.496+/-0.002 ...
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We demonstrate that high-resolution multidimensional solid state NMR methods can be used to correlate many backbone and side chain chemical shifts for hydrated micro-crystalline U-13C,15N Basic Pancreatic Trypsin Inhibitor (BPTI), using a field strength of 800 MHz for protons, magic angle sample spinning rates of 20 kHz and proton decoupling field strengths of 140 kHz. Results from two homonucl...
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We describe a new experiment for measuring homonuclear-decoupled anisotropic chemical shift patterns in doubly 13C-labeled compounds under magic-angle spinning. The experiment combines a pair of selective and non-selective 180 degrees pulses to suppress the 13C-13C scalar and dipolar interactions. This is combined with the recently developed SUPER technique to recouple the chemical shift anisot...
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The use of uniformly 13C,15N-labeled RNA has greatly facilitated structural studies of RNA oligonucleotides by NMR. Application of similar methodologies for the study of DNA has been limited, primarily due to the lack of adequate methods for sample preparation. Methods for both chemical and enzymatic synthesis of DNA oligonucleotides uniformly labeled with 13C and/or 15N have been published, bu...
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A new NMR technique for determining long-range 1H-19F distances in solids is demonstrated. Using a modified rotational-echo double resonance (REDOR) sequence involving 1H homonuclear decoupling and composite 19F pulses, we show that it is possible to determine 1H-19F distances to approximately 8 A. The detrimental effect of the large 19F chemical shift to REDOR dephasing is partially compensate...
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ژورنال
عنوان ژورنال: Solid State Nuclear Magnetic Resonance
سال: 2012
ISSN: 0926-2040
DOI: 10.1016/j.ssnmr.2012.06.001